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Título: Identification of Bacillus thuringiensis strains for the management of lepidopteran pests.
Autor: PINHEIRO, D. H.
VALICENTE, F. H.
Afiliación: DANIELE HELOÍSA PINHEIRO; FERNANDO HERCOS VALICENTE, CNPMS.
Año: 2021
Referencia: Neotropical Entomology, v. 50, p. 804-811, 2021.
Descripción: Bacillus thuringiensis (Bt)-based bioinsecticides and transgenic plants expressing proteins with insecticidal activity (Cry and Vip) have been successfully used in several integrated pest management programs worldwide. Lepidoptera comprise some of the most economically important insect pests of the major agricultural crops. In this study, the toxicity of 150 Btstrains was evaluated against Helicoverpa armigera (Hübner) larvae. Eight strains (426, 520B, 1636, 1641, 1644, 1648, 1657 and 1658) showed high insecticide activity against H. armigera and were therefore tested against Anticarsia gemmatalis (Hübner), Spodoptera cosmioides (Walker), Chrysodeixis includens (Walker), and Diatraea saccharalis (Fabricius) larvae. Our results showed that most of the Bt strains were also toxic to these lepidopteran species. The biochemical and molecular analyses of these strains revealed that they had a similar protein profile; however, their cry and vip gene contents were variable. In addition, the median lethal concentration (LC50) of the selected strains indicated that the strains 1636, 1641, and 1658 were the most effective against H. armigera, showing LC50 values of 185.02, 159.44, and 192.98 ng/cm2 , respectively. Our results suggest that the selected Bt strains have great potential to control the lepidopteran pests H. armigera, A. gemmatalis, D. saccharalis, S. cosmioides, and C. includes.
Thesagro: Praga de Planta
Controle Biológico
Bactéria
Gene
Inseto
NAL Thesaurus: Pest control
Insects
Entomopathogenic bacteria
Palabras clave: Bioinseticida
Bioinsecticide
DOI: https://doi.org/10.1007/s13744-021-00896-w
Tipo de Material: Artigo de periódico
Acceso: openAccess
Aparece en las colecciones:Artigo em periódico indexado (CNPMS)

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