Title:	Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.
Authors:	BIANCHI, S. R. AMARAL, C. D. B. do SILVA, C. S. da NOGUEIRA, A. R. de A.
Affiliation:	SILMARA ROSSANA BIANCHI, CNPS; Clarice Dias Britto do Amaral, UFPR; Caroline Santos da Silva, UFSCar; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.
Date Issued:	2017
Citation:	Journal of the Brazilian Chemical Society, v. 28, n. 12, p. 2357-2364, 2017.
Description:	Determination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.
NAL Thesaurus:	selenium
Keywords:	Bovine semen Cattle fertility
DOI:	http://dx.doi.org/10.21577/0103-5053.20170089
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPS)