Título:	Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens.
Autoria:	HUL, L. M. IBELLI, A. M. G. SAVOLDI, I. R. MARCELINO, D. E. P. FERNANDES, L. T. PEIXOTO, J. O. CANTAO, M. E. HIGA, R. H. GIACHETTO, P. F. COUTINHO, L. L. LEDUR, M. C.
Afiliação:	LUDMILA MUDRI HUL, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; IGOR RICARDO SAVOLDI, UDESC; DÉBORA ESTER PETRY MARCELINO, FACULDADE DE CONCÓRDIA; LANA TEIXEIRA FERNANDES; JANE OLIVEIRA PEIXOTO, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; MAURICIO EGIDIO CANTAO, CNPSA; ROBERTO HIROSHI HIGA, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; MONICA CORREA LEDUR, CNPSA.
Ano de publicação:	2021
Referência:	Scientific Reports, v. 11, n. 1, p. 1-13. 2021.
Conteúdo:	Locomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans.
Thesagro:	Frango Frango de Corte
NAL Thesaurus:	Broiler chickens Gene expression Quantitative polymerase chain reaction Transcriptome
Palavras-chave:	Expressão gênica Sequenciamento de RNA Cartilagem da cabeça do fêmur Separação da cabeça femoral Análises de expressão diferencial Reação da cadeia de polimerase Transcriptoma da cartilagem do fêmur Differential expression analysis
Digital Object Identifier:	https://doi.org/10.1038/s41598-021-97306-3
Notas:	Article number: 17965.
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPSA)