Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135377
Research center of Embrapa/Collection: Embrapa Suínos e Aves - Artigo em periódico indexado (ALICE)
Date Issued: 2021
Type of Material: Artigo em periódico indexado (ALICE)
Authors: HUL, L. M.
IBELLI, A. M. G.
SAVOLDI, I. R.
MARCELINO, D. E. P.
FERNANDES, L. T.
PEIXOTO, J. O.
CANTAO, M. E.
HIGA, R. H.
GIACHETTO, P. F.
COUTINHO, L. L.
LEDUR, M. C.
Additional Information: LUDMILA MUDRI HUL, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; IGOR RICARDO SAVOLDI, UDESC; DÉBORA ESTER PETRY MARCELINO, FACULDADE DE CONCÓRDIA; LANA TEIXEIRA FERNANDES; JANE OLIVEIRA PEIXOTO, UNIVERSIDADE ESTADUAL DO CENTRO-OESTE, GUARAPUAVA; MAURICIO EGIDIO CANTAO, CNPSA; ROBERTO HIROSHI HIGA, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; MONICA CORREA LEDUR, CNPSA.
Title: Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens.
Publisher: Scientific Reports, v. 11, n. 1, p. 1-13. 2021.
Language: Ingles
Notes: Article number: 17965.
Keywords: Expressão gênica
Sequenciamento de RNA
Cartilagem da cabeça do fêmur
Separação da cabeça femoral
Análises de expressão diferencial
Reação da cadeia de polimerase
Transcriptoma da cartilagem do fêmur
Differential expression analysis
Description: Locomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans.
Thesagro: Frango
Frango de Corte
NAL Thesaurus: Broiler chickens
Gene expression
Quantitative polymerase chain reaction
Transcriptome
Data Created: 2021-10-15
Appears in Collections:Artigo em periódico indexado (CNPSA)

Files in This Item:
File Description SizeFormat 
final9712.pdf1,35 MBAdobe PDFThumbnail
View/Open

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace