Title:	Fast, simple and cheap: method modified from conventional cultivation for tuberculosis diagnosis allows seeding on Löwenstein-Jensen of any swab-embedded pulmonary samples decontaminated with sodium hydroxide.
Authors:	COSTA, R. R. da SILVA, M. R. AUGUSTO, C. J. LEITE, I. C. G.
Affiliation:	RONALDO RODRIGUES DA COSTA, Universidade Federal de Juiz de Fora; MARCIO ROBERTO SILVA, CNPGL; CLAUDIO JOSE AUGUSTO, Fundação Ezequiel Dias; ISABEL CRISTINA GONÇALVES LEITE, Universidade Federal de Juiz de Fora.
Date Issued:	2021
Citation:	Transactions of The Royal Society of Tropical Medicine and Hygiene, trab162, 2021.
Description:	Background: Few tuberculosis (TB) control programmes in low-income countries have access to culture facilities in their primary care diagnostic centres and this scenario may have worsened with the coronavirus disease 2019 pandemic. Thus the aim was to develop and evaluate a simpler TB test that allows seeding on Löwenstein?Jensen (LJ) medium of several swab-embedded samples decontaminated with sodium hydroxide (NaOH). Methods: A cotton swab containing each sample was decontaminated in NaOH before being dipped into aslightly acidic solution to neutralize the pH in order to allow the culture to develop on LJ medium. Samples (n=543) from suspected or confirmed pulmonary TB were analysed in two phases: standardization (n=167) and evaluation of the study method (n=376). Results: The study method showed sensitivity >95% and specificity >93% using Ogawa?Kudoh (OK) and modified Petroff (MP) as standards and was comparable to MP-LJ (p>0.05) and slightly superior to OK (p=0.03) for sputum culture and more comprehensive than the latter for other pulmonary specimens. Conclusions: This article reports a more comprehensive, simpler and less costly method for diagnosing TB inthe laboratory with fewer economic resources and biosafety equipment. Thus a patent application was filed (BR1020190103841).
Thesagro:	Tuberculose Doença Animal
DOI:	https://doi.org/10.1093/trstmh/trab162
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)