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|Research center of Embrapa/Collection:||Embrapa Mandioca e Fruticultura - Artigo em periódico indexado (ALICE)|
|Type of Material:||Artigo em periódico indexado (ALICE)|
|Authors:||BEZERRA, Y. C. dos A.|
MARQUES, J. P. R.
STIPP, L. C. L.
ATTÍLIO, L. B.
ASTUA, J. de F.
MOURÃO FILHO, F. de A. A.
|Additional Information:||YANE CAROLINE DOS ANJOS BEZERRA, USP; JOÃO PAULO RODRIGUES MARQUES, USP; LILIANE CRISTINA LIBORIO STIPP, USP; LÍSIA BORGES ATTÍLIO, USP; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, USP.|
|Title:||How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters.|
|Publisher:||Revista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021.|
|Description:||New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.|
|Appears in Collections:||Artigo em periódico indexado (CNPMF)|
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