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|Title:||Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples.|
OKINO, C. H.
AZEVEDO, B. T.
KATIKI, L. M.
OLIVEIRA, M. C. de S.
VERCESI FILHO, A. E.
|Affiliation:||RODRIGO GIGLIOTI, IZ|
CINTIA HIROMI OKINO, CPPSE
BIANCA TAINA AZEVEDO, IZ
GUNTA GUTMANIS, IZ
LUCIANA MORITA KATIKI, IZ
MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE
ANIBAL EUGENIO VERCESI FILHO, IZ.
|Citation:||Food Chemistry: Molecular Sciences, v.3, 2021, 100055.|
|Description:||The rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products.|
Real time PCR
|Type of Material:||Artigo de periódico|
|Appears in Collections:||Artigo em periódico indexado (CPPSE)|