Título:	Prevalence and molecular characterization of carbapenem-resistant Enterobacterales in patients from a public referral hospital in a non-metropolitan region of Brazil during and post the SARS-CoV-2 pandemic.
Autor:	FOCHAT, R. C. ARAÚJO, A. C. de L. PEREIRA JÚNIOR, O. dos S. P. SILVÉRIO, M. S. NASSAR, A. F. de C. JUNQUEIRA, M. de L. SILVA, M. R. GARCIA, P. G.
Afiliación:	ROMÁRIO COSTA FOCHAT, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ANA CLARA DE LELIS ARAÚJO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; OLAVO DOS SANTOS PEREIRA JÚNIOR, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; MARCELO SILVA SILVÉRIO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ALESSANDRA FIGUEIREDO DE CASTRO NASSAR, INSTITUTO BIOLÓGICO; MARIA DE LOURDES JUNQUEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; MARCIO ROBERTO SILVA, CNPGL; PATRÍCIA GUEDES GARCIA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA.
Año:	2024
Referencia:	Brazilian Journal of Microbiology, v. 55, p. 3873-3884, 2024.
Descripción:	Antimicrobial resistance (AMR) poses a global threat, with carbapenem-resistant Enterobacterales (CRE) representing a significant concern due to limited therapeutic options. This study investigated the prevalence of carbapenemase genes in CRE strains isolated from tracheal aspirates of patients at a Brazilian university hospital between January 2020 and August 2023. Bacterial identification was conducted using MALDI-TOF, while carbapenemase genes were detected by qPCR. Demographic and clinical data were collected, and univariate analysis was performed using the chi-square test (p<0.05). Variables with p≤0.10 were further investigated using the chi-square test for linear trend, along with stratified analysis. Out of 1,133 samples, 111 (9.79%) showed CRE growth, with 46 isolates included in the final sample, predominantly comprising Klebsiella pneumoniae (65.21%) and Serratia marcescens (19.57%). The blaKPC gene was prevalent (78.26%), while blaNDM was detected in 21.74% of cases. The identified population was predominantly male (67.39%), elderly (69.57%), white (56.52%), unmarried (63.04%), and had a low level of education (56.52%). Most patients (69.57%) were in the intensive care unit and remained hospitalized for more than 30 days (76.08%). There was a significant inverse trend between Klebsiella pneumoniae and age (p=0.045), as well as a direct linear trend between blaNDM and the annual increase in COVID-19 cases in Brazil (p=0.050). A high probability of finding non-Klebsiella pneumoniae bacteria was observed in patients with prolonged hospital stays, independent of COVID-19 (p=0.006) and the type of resistance genes (p=0.020). The persistent prevalence of CRE, especially with blaKPC, underscores the urgency of effective control measures.
Thesagro:	Bactéria
NAL Thesaurus:	Serratia marcescens Klebsiella pneumoniae subsp. pneumoniae
Palabras clave:	Antimicrobial resistance Resistência antimicrobiana Aspirado traqueal
DOI:	https://doi.org/10.1007/s42770-024-01531-7
Tipo de Material:	Artigo de periódico
Acceso:	openAccess
Aparece en las colecciones:	Artigo em periódico indexado (CNPGL)