Título:	Genetic transformation of common beans (Phaseolus vulgaris): achievements and challenges.
Autoria:	MOURA, M. da C. PINHEIRO, P. V. VIANELLO, R. P. SOUSA, N. L. de FARIA, J. C. de ARAGÃO, F. J. L.
Afiliação:	MATHEUS DA COSTA MOURA, UNIVERSITY OF BRASILIA; PATRICIA VALLE PINHEIRO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF; NATÁLIA LIMA DE SOUSA; JOSIAS CORREA DE FARIA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN.
Ano de publicação:	2024
Referência:	Agriculture, v. 14, 2060, 2024.
Conteúdo:	Genetic transformation is a valuable tool for the development of plant varieties with desirable traits that are present in the species germplasm with low genetic variability, i.e., resistance to pests and diseases and nutritional improvements. Although transgenic and edited crops have been successfully obtained for many plant species, it remains difficult for common beans (Phaseolus vulgaris), due to their recalcitrance to in vitro regeneration. This review discusses various methods employed, such as Agrobacterium-mediated transformation, biolistic (particle bombardment), and hairy root systems, noting their respective efficiencies and limitations. While there has been progress, including the development of the first transgenic common bean cultivar approved for commercialization (Embrapa 5.1), the article emphasizes the need for improved protocols and techniques for more efficient genetic transformation. It also touches upon the potential of gene editing technologies like CRISPR/Cas9 in overcoming existing challenges and facilitating the development of resilient bean varieties.
Thesagro:	Leguminosae Agrobacterium Tumefaciens Melhoramento Genético Vegetal Organismo Transgênico
NAL Thesaurus:	Plant breeding In vitro regeneration Recalcitrant species
Palavras-chave:	Genetically modified organism Transgenic Gene editing Biolistic Hairy root
ISSN:	2077-0472
Digital Object Identifier:	https://doi.org/10.3390/agriculture14112060
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPAF)