Título:	New and efficient AS-PCR molecular marker for selection of coffee resistant to coffee leaf rust.
Autoria:	ALMEIDA, D. P. de CARNEIRO, D. G. CAIXETA, E. T. ALVES, D. R. ZAMBOLIM, L.
Afiliação:	DÊNIA PIRES DE ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; DEISY GUIMARÃES CARNEIRO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA MOURA, CNPCA; DANÚBIA RODRIGUES ALVES, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA.
Ano de publicação:	2024
Referência:	Crop Breeding and Applied Biotechnology, v. 24, n. 4, e498424410, 2024.
Conteúdo:	Coffee leaf rust, caused by Hemileia vastatrix, is the most important disease affecting Coffea arabica. The use of molecular markers through marker-assisted selection (MAS) is an effective approach to develop cultivars with multiple and durable resistance, which is economically and environmentally beneficial for this disease control. In this work, a new molecular marker based on allele-specific polymerase chain reaction (AS-PCR) associated with the quantitative trait locus (QTL) of the linkage group 5 (LG5), which confers resistance to races I, II and pathotype 001 of H. vastatrix, was developed. The CaRHv10-AS marker was validated in a segregating genetic mapping population of 247 F2 genotypes and demonstrated selection efficiency greater than 97% for genotypes with resistance to three races. Thus, this work provides a fast, robust, and affordable molecular marker for use in MAS, facilitating the development of coffee plants with multiple resistance and ensuring sustainable coffee production.
Thesagro:	Hemileia Vastatrix
NAL Thesaurus:	Plant breeding Sustainable agriculture Marker-assisted selection
Digital Object Identifier:	https://doi.org/10.1590/1984-70332024v24n4a47
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (SAPC)