Title:	Activity of Sesquiterpene Lactones and Umbelliferone From <i>Campovassouria cruciata</i> on SARS‐CoV‐2 Replication and on the Release of Pro‐Inflammatory Cytokines in Lung Cells
Authors:	RABELO, M. B. de O. FREITAS, K. M. OLIVEIRA, L. C. de FRADE, A. C. M. S. FILHO, J. D. de HEIDEN, G. FERNANDES, G. W. SILVA, I. T. TEIXEIRA, M. M. BRAGA, F. C. PÁDUA, R. M.
Affiliation:	MARIA BEATRIZ DE O. RABELO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; KÁTIA M. FREITAS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; LEONARDO C. DE OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ALESSANDRA C. M. FRADE, UNIVERSIDADE FEDERAL DE MINAS GERAIS; JOSÉ D. DE S. FILHO, UNIVERSIDADE FEDERAL DE OURO PRETO; GUSTAVO HEIDEN, CPACT; GERALDO W. FERNANDES, UNIVERSIDADE FEDERAL DE MINAS GERAIS; IZABELLA THAÍS SILVA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; MAURO MARTINS TEIXEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; FERNÃO C. BRAGA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; RODRIGO M. PÁDUA, UNIVERSIDADE FEDERAL DE MINAS GERAIS.
Date Issued:	2025
Citation:	Chemistry and Biodiversity, e202402824, 2025.
Description:	The investigation of natural products is a valid strategy to identify compounds active against coronaviruses. We herein report the screening of Baccharis and Campovassouria plant species against the murine betacoronavirus murine hepatitis virus-3 (MHV-3), assays that can be carried out in BSL-2 facilities. These genera occur exclusively in the Americas and are a source of secondary metabolites with antiviral and anti-inflammatory activity. Campovassouria cruciata emerged as the most active species from the screening, and its phytochemical investigation afforded the discovery of a new isomer of leptocarpin, 3-epi-leptocapin (1), in addition to the sesquiterpenes lactones leptocarpin (2) and arturin (3), along with umbelliferone (4). The structures were elucidated by extensive mono- and two-dimensional 1H and 13C NMR data analysis. The antiviral activity of the isolated compounds was also assayed in Calu-3 cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). All compounds inhibited SARS-CoV-2 replication, with a reduction in viral load of approximately 1.7-2.0 log when tested at 7.5 µM. To evaluate potential anti-inflammatory activity, compounds were tested in A549 cells stimulated with IFN-γ and TNF-α. Both 3-epi-leptocarpin (1) and umbelliferone (4) inhibited interleukin-6 (IL-6) and IL-8 release significantly. Compound 1 reduced the production of reactive oxygen species (ROS) in A549 cells, as assessed by flow cytometry using DCFH-DA, whereas 4 exhibited ROS values seven-fold higher than the basal level. Our results highlight C. cruciata as a source of promising bioactive compounds, which deserve future investigations to explore their potential for the development of new therapeutic agents against inflammatory and viral diseases.
Thesagro:	Antiinflamatório
NAL Thesaurus:	Antiviral agents Anti-inflammatory agents Severe acute respiratory syndrome coronavirus
Keywords:	Lactonas sesquiterpênicas Sesquiterpene lactones Coronavírus 2 da síndrome respiratória aguda grave
DOI:	10.1002/cbdv.202402824
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPACT)