Título:	Development of transgenic cassava cultivars from northeastern Brazil.
Autoria:	IBRAHIM, A. B. HEREDIA, F. F. BARBOSA, C. P. MACHADO, T. F. CAMPOS, F. A. P.
Afiliação:	Abdulrazak Baba Ibrahim, UFC; Fabiola Fernandes Heredia, UFC; Camila P. Barbosa, UFC; Terezinha Feitosa Machado, CNPAT; Francisco de Assis Paiva Campos, UFC.
Ano de publicação:	2007
Referência:	In: INTERNATIONAL MEETING ON CASSAVA BREEDING, BIOTECHNOLOGY AND ECOLOGY, 1., 2007, Brasília, DF. Proceedings... Brasília, DF: UNB, 2007.
Páginas:	p.115-130
Conteúdo:	Following the establishment ofa cyclic system of somatic embryogenesis n excess of 20 independent friable embryogenic callus (FEC) lines originating from different embryogenic cycles were established when fragments of somatic embryos were cultured on GD medium containing 2% sucrose Supplemented with 50uM picloram. Histodifierentiation of these embryogenic structures was achieved when the tissues were transfeflfll to MS medium devoid of growth regulators or to a medium supplemented withNAA or 2, 4-D. Differentiation of the tissues demonstrated that somatic embryos can be recovered at the rate of 450 embryos per gram of FEC tissues over 8 period ofsix weeks. Histodiflerentiation of the FEC resulted in malformed cotyledon-Stage embryos, which when subjected to maturation, germina- tion and organogenesis using three difierent strategies did not regenerate into plants. Histological cuttings of the embryogenic tissues confirmed the malformation and absence of meristem. Up to 50mg L" of kanamycin is required to arrest embryogenic potential of green cotyledons from somatic embryos. While 50 to 60mg L" of paromomycin arrested the proliferation and histodiflerentiation of FEC, 5mg L" of the antibiotic was sufficient to arrest the proliferation of the cells in liquid medium.
NAL Thesaurus:	Cassava Transgenic plants
Tipo do material:	Artigo em anais e proceedings
Acesso:	openAccess
Aparece nas coleções:	Artigo em anais de congresso (CNPAT)