Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/515320
Research center of Embrapa/Collection: Embrapa Caprinos e Ovinos - Artigo em periódico indexado (ALICE)
Date Issued: 2000
Type of Material: Artigo em periódico indexado (ALICE)
Authors: ALVES, F. S. F.
LEFEBVRE, R. B.
PROBERT, W.
Additional Information: FRANCISCO SELMO FERNANDES ALVES, CNPC; Rance B. LeFebvre, University of California, Davis/Department of Veterinary Microbiology, Immunology and Pathology, 95616-Davis, CA, USA; William Probert, University of California, Davis/Department of Veterinary Microbiology, Immunology and Pathology, 95616-Davis, CA, USA.
Title: Amino acid sequences of proteins from Leptospira serovar pomona.
Publisher: Memórias do Instituto Oswaldo Cruz, p. 95, n. 4, p. 503-504, jul./ago. 2000.
Language: en
Keywords: Sequência
Sequence.
Description: This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45kDa. In situ digestion of 40kDa molecular weight protein was accomplished using cyanogen bromide. The 32kDa protein generated two fragments, one of 21kDa and another of 10kDa that yielded five residues. A fragment of 24 kDa that yielded nineteen residues of amino acids was obtained from 45 kDa protein. A fragment with a molecular weight of 20 kDa, yielding a twenty amino acids sequence from the 40kDa protein.
Thesagro: Aminoácido
Proteína
Leptospira pomona
Leptospirose
Doença animal
NAL Thesaurus: Leptospira
Proteins
Amino acids
Animal diseases.
Data Created: 2000-08-07
Appears in Collections:Artigo em periódico indexado (CNPC)

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