Title:	Isolation, culture and cryopreservation of umbilical cord cells, adipocytes cells and fibroblasts from bovine fetuses.
Authors:	OLIVEIRA, V. V. G. SILVA, C. G. PIRES, N. L. CUNHA, E. R. GUIMARÃES, C. O. SERENO, J. R. B. MARTINS, C. F.
Affiliation:	VINICIUS VASCONCELOS GOMES DE OLIVEIRA, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; CAROLINA GONZALES DA SILVA; NAYARA LUIZ PIRES; ELISA RIBEIRO DA CUNHA; JOSE ROBSON BEZERRA SERENO, CPAC; CARLOS FREDERICO MARTINS, CPAC.
Date Issued:	2010
Citation:	Acta Scientiae Veterinariae, v. 38, supl. 2, p. s803, 2010.
Description:	Considering the importance of bovine cloning, this work had the following objectives: a-evaluate the possibility of isolation and culture of umbilical cord cells, adipocytes cells and fibroblasts from bovine fetuses b-test the effect of two cryopreservation solutions containing DMSO or DMF to protect the cells, c-determine if the trypan blue dye is better than the fluorescent probes (carboxyfluorescein acetate and propidium iodide) for identify the viability of frozen cells.
Thesagro:	Criopreservação Feto Bovino
NAL Thesaurus:	Cryopreservation Umbilical cord Adipocytes Fibroblasts
Keywords:	Cordão umbilical
Notes:	Edição dos resumos da 25. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, PE, 2010. Abstract 205.
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CPAC)