Title:	Ectopic expression of a Meloidogyne incognita dorsal gland protein in tobacco accelerates the formation of the nematode feeding site.
Authors:	LIMA E SOUZA, D. dos S de SOUZA JUNIOR, J. D. A. de GROSSI-DE-SÁ, M. ROCHA, T. L. FRAGOSO, R. da R. BARBOSA, A. E. A. de D. OLIVEIRA, G. R. de NAKASU, E. Y. T. SOUSA, B. A. de PIRES, N. F. DUSI, D. M. de A. CARNEIRO, R. M. D. G. PINTO, E. R. de C. ALMEIDA-ENGLER, J. de ENGLER, G. MARTINS-DE-SÁ, C. SA, M. F. G. de
Affiliation:	DJAIR DOS SANTOS DE LIMA E SOUZA, UNIVERSIDADE DE BRASÍLIA JOSÉ DIJAIR ANTONINO DE SOUZA JUNIOR, UNIVERSIDADE DE BRASÍLIA MAÍRA GROSSI-DE-SÁ, UNIVERSIDADE DE BRASÍLIA THALES LIMA ROCHA, CENARGEN RODRIGO DA ROCHA FRAGOSO, CPAC AULUS ESTEVÃO ANJOS DE DEUS BARBOSA, UNIVERSIDADE CATÓLICA DE BRASÍLIA GUSTAVO RAMOS DE OLIVEIRA ERICH YUKIO TEMPEL NAKASU, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL BRUNA DE ARAUJO SOUSA, UNIVERSIDADE CATÓLICA DE BRASÍLIA NATÁLIA FAUSTINO PIRES DIVA MARIA DE ALENCAR DUSI, CENARGEN REGINA MARIA DECHECHI GOMES CARNEIRO, CENARGEN EDUARDO ROMANO DE CAMPOS PINTO, CENARGEN JANICE DE ALMEIDA ENGLER, INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT GILBERT ENGLER, INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT CEZAR MARTINS-DE-SÁ, UNIVERSIDADE DE BRASÍLIA MARIA FATIMA GROSSI DE SA, CENARGEN.
Date Issued:	2011
Citation:	Plant Science, v. 180, p. 276-282, 2011.
Description:	Meloidogyne spp., plant-parasitic nematodes present worldwide, are intensively studied because of the damage caused to a large variety of agronomically important crops. Several reports indicate that proteins from the Meloidogyne spp. dorsal gland might play an important role to allow proper establishment of a functional nematode feeding site. The precise role of these proteins in the process of feeding cell development is unknown. To gain insights into the function of these secreted M. incognita proteins, we constitutively (ectopically) expressed the nematodes dorsal gland protein 7E12 in tobacco plants. It was found that the number of galls at 8 and 16 days after nematode infection was significantly higher in transgenic plants compared to control plants. Eggs from nematodes in transgenic plants hatched faster than those in control plants. Histological analysis of nematode induced galls in transgenic plants clearly shows a different morphology. Giant feeding cells harbor more vacuoles and an increased amount of cell wall invaginations, while neighboring cells surrounding feeding cells are more numerous. These results suggest that the presence of the 7E12 protein in tobacco accelerates gall formation. This assumption is supported by our data illustrating faster gall formation and egg eclosion in transgenic plants.
Thesagro:	Fumo Nematóide
DOI:	https://doi.org/10.1016/j.plantsci.2010.09.003
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CENARGEN)