Título:	Identification of a novel α-L-arabinofuranosidase gene associated with mealiness in apple.
Autoria:	NOBILE, P. M. WATTEBLED, F. QUECINI, V. M. GIRARDI, C. L. LORMEAU, M. LAURENS, F.
Afiliação:	PAULA MACEDO NOBILE, INRA; FABRICE WATTEBLED, UNIVERSITÉ DES SCIENCES ET TECHNOLOGIES DE LILLE; VERA MARIA QUECINI, CNPUV; CESAR LUIS GIRARDI, CNPUV; MARYLINE LORMEAU, INRA; FRANÇOIS LAURENS, INRA.
Ano de publicação:	2011
Referência:	Journal of Experimental Botany, London, v. 62, v. 12, p. 4309-4321, 2011.
Conteúdo:	In order to investigate the genetic bases of the physiological syndrome mealiness that causes abnormal fruit softening and juice loss in apples, an integrative approach was devised, consisting of sensory, instrumental, biochemical, genetic, and genomic methods. High levels of activity of a-L-arabinofuranosidase (a-AFase), a hydrolase acting on the pectic component of the cell walls, were found in individuals exhibiting the mealiness phenotype in a segregating population. The expression levels of the previously uncharacterized apple AF gene MdAF3 are higher in fruits from plants consistently showing mealiness symptons and high a-AFase activity. The transcription of MdAF3 is differentially regulated in distinct genomic contexts and appears to be independent of ethylene. Thus, it is likely to be controlled by endogenous developmental mechanisms associated with fruit ripening. The use of integrative approaches has allowed the identification of a novel contributor to the mealiness phenotype in apple and it has been possible to overcome the problems posed by the unavailability of near-isogenic lines to dissect the genetic bases of a complex physiological trait in woody perennial species.
Thesagro:	Fruticultura Maçã Genética Distúrbio fisiológico
Palavras-chave:	Expressão gênica
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPUV)