Title:	Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specific primers and multiplex PCR.
Authors:	ARAÚJO, E. R. COSTA, J. R. FERREIRA, M. A. S. V. QUEZADO-DUVAL, A. M.
Affiliation:	ALICE MARIA QUEZADO DUVAL, CNPH.
Date Issued:	2012
Citation:	Journal of Applied Microbiology, Oxford, Sept. 2012.
Description:	To establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification.
Thesagro:	Bactéria Doença Identificação
NAL Thesaurus:	Xanthomonas
Keywords:	Detecção PCR
ISSN:	1364-5072
DOI:	10.1111/j.1365-2672.05431.x
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPH)