Title:	Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (delta-abcBA) Protects against Wild Type Brucella ovis in Rams.
Authors:	SILVA, A. P. C. MACÊDO, A. A. COSTA, L. F. ROCHA, C. E. GARCIA, L. N. N. FARIAS, J. R. D. GOMES, P. P. R. TEIXEIRA, G. C. FONSECA, K. W. J. MAIA, A. R. F. NEVES, G. G. ROMÃO, E. L. SILVA, T. M. A. MOL, J. P. S. OLIVEIRA, R. M. ARAÚJO, M. S. S. NASCIMENTO, E. F. MARTINS-FILHO, O. A. BRANDAO, H. de M. PAIXÃO, T. A. SANTOS, R. L.
Affiliation:	Ana P. C. SILVA, UFMG; Auricélio A. Macêdo, UFMG; Luciana F. Costa, UFMG; Cláudia E. Rocha, UFMG; Luize N. N. Garcia, UFMG; Jade R. D. Farias, UFMG; Priscilla P. R. Gomes, UFMG; Gustavo C. Teixeira, UFMG; Kessler W. J. Fonseca, UFMG; Andréa R. F. Maia, UFMG; Gabriela G. Neves, UFMG; Everton L. Romão, UFMG; Teane M. A. Silva, UFMG; Juliana P. S. Mol, UFMG; Renata M. Oliveira, Universidade Estadual do Maranhão; Márcio S. S. Araújo, Fundação Oswaldo Cruz, MG; Ernane F. Nascimento, UFMG; Olindo A. Martins-Filho, Fundação Oswaldo Cruz, MG; HUMBERTO DE MELLO BRANDAO, CNPGL; Tatiane A. Paixão, UFMG; Renato L. Santos, UFMG.
Date Issued:	2015
Citation:	Plos One, v. 10, n. 8, e013865, 2015.
Description:	This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.
NAL Thesaurus:	animal health
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)