Title:	Enrichment and acclimation of an anaerobic mesophilic microorganism?s inoculum for standardization of BMP assays.
Authors:	STEINMETZ, R. L. R. MEZZARI, M. P. SILVA, M. L. B. da KUNZ, A. AMARAL, A. C. TÁPPARO, D. C. SOARES, H. M.
Affiliation:	RICARDO LUIS RADIS STEINMETZ, CNPSA; MELISSA PAOLA MEZZARI, UNOESC; MARCIO LUIS BUSI DA SILVA, CNPSA; AIRTON KUNZ, CNPSA; ANDRÉ CESTONARO DO AMARAL, UNIOESTE; DEISI CRISTINA TÁPPARO, UNIOESTE; HUGO MOREIRA SOARES, UFSC.
Date Issued:	2016
Citation:	Bioresource Technology, v. 219, p. 21-28, 2016.
Description:	Appropriate enrichment of anaerobic microorganism?s consortium is crucial for accurate biochemical methane potential (BMP) assays. An alternative method to produce and maintain a mesophilic methanogenic inoculum was demonstrated. Three sources of inoculum were mixed and acclimated for 857 days in order to reach steady conditions (pH = 7.90 ± 0.46; VS/TS > 50%; VFA/alkalinity = 0.16 ± 0.04 gAcetic Acid/gCaCO3 ). Biogas yield >80% was obtained after 70 days of inoculum acclimation in comparison to standard cellulose (>600 mLN/gVS). Methanogen community analysis based on 16S rDNA of the inoculum revealed Archaea concentration of 3 1012 gene copies/g (Methanobacteriales 8 1010; Methanomicrobiales 8 1010; and Methanosarcinales 4 1011 gene copies/g). The proposed method for development and maintenance of microorganism enrichment inoculum demonstrates consistent BMP data which is a requirement for dependable prediction of biogas production at field scale operations.
Thesagro:	Dejeto Biogás
NAL Thesaurus:	Anaerobic digestion Waste treatment
Keywords:	Digestão anaeróbica
DOI:	http://dx.doi.org/10.1016/j.biortech.2016.07.031
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPSA)