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Título: | Enrichment and acclimation of an anaerobic mesophilic microorganism?s inoculum for standardization of BMP assays. |
Autor: | STEINMETZ, R. L. R. MEZZARI, M. P. SILVA, M. L. B. da KUNZ, A. AMARAL, A. C. TÁPPARO, D. C. SOARES, H. M. |
Afiliación: | RICARDO LUIS RADIS STEINMETZ, CNPSA; MELISSA PAOLA MEZZARI, UNOESC; MARCIO LUIS BUSI DA SILVA, CNPSA; AIRTON KUNZ, CNPSA; ANDRÉ CESTONARO DO AMARAL, UNIOESTE; DEISI CRISTINA TÁPPARO, UNIOESTE; HUGO MOREIRA SOARES, UFSC. |
Año: | 2016 |
Referencia: | Bioresource Technology, v. 219, p. 21-28, 2016. |
Descripción: | Appropriate enrichment of anaerobic microorganism?s consortium is crucial for accurate biochemical methane potential (BMP) assays. An alternative method to produce and maintain a mesophilic methanogenic inoculum was demonstrated. Three sources of inoculum were mixed and acclimated for 857 days in order to reach steady conditions (pH = 7.90 ± 0.46; VS/TS > 50%; VFA/alkalinity = 0.16 ± 0.04 gAcetic Acid/gCaCO3 ). Biogas yield >80% was obtained after 70 days of inoculum acclimation in comparison to standard cellulose (>600 mLN/gVS). Methanogen community analysis based on 16S rDNA of the inoculum revealed Archaea concentration of 3 1012 gene copies/g (Methanobacteriales 8 1010; Methanomicrobiales 8 1010; and Methanosarcinales 4 1011 gene copies/g). The proposed method for development and maintenance of microorganism enrichment inoculum demonstrates consistent BMP data which is a requirement for dependable prediction of biogas production at field scale operations. |
Thesagro: | Dejeto Biogás |
NAL Thesaurus: | Anaerobic digestion Waste treatment |
Palabras clave: | Digestão anaeróbica |
DOI: | http://dx.doi.org/10.1016/j.biortech.2016.07.031 |
Tipo de Material: | Artigo de periódico |
Acceso: | openAccess |
Aparece en las colecciones: | Artigo em periódico indexado (CNPSA) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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final8317.pdf | 409,98 kB | Adobe PDF | Visualizar/Abrir |