Title:	In silico phylogenetic and virulence gene profile analyses of avian pathogenic Escherichia coli genome sequences.
Authors:	ROJAS, T. C. G. MALUTA, R. P. KOENIGKAN, L. V. SILVEIRA, W. D. da
Affiliation:	THAÍS C. G. ROJAS, IB/Unicamp; RENATO P. MALUTA, IB/Unicamp; LUCIANO VIEIRA KOENIGKAN, CNPTIA; WANDERLEY DIAS DA SILVEIRA, IB/Unicamp.
Date Issued:	2014
Citation:	Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 34, n. 2, p. 129-133, fev. 2014.
Description:	Avian pathogenic Escherichia coli (APEC) infections are responsible for significant losses in the poultry industry worldwide. A zoonotic risk has been attributed to APEC strains because they present similarities to extraintestinal pathogenic E. coli (ExPEC) associated with illness in humans, mainly urinary tract infections and neonatal meningitis. Here, we present in silico analyses with pathogenic E. coli genome sequences, including recently available APEC genomes. The phylogenetic tree, based on multi-locus sequence typing (MLST) of seven housekeeping genes, revealed high diversity in the allelic composition. Nevertheless, despite this diversity, the phylogenetic tree was able to cluster the different pathotypes together. An in silico virulence gene profile was also determined for each of these strains, through the presence or absence of 83 well-known virulence genes/traits described in pathogenic E. coli strains. The MLST phylogeny and the virulence gene profiles demonstrated a certain genetic similarity between Brazilian APEC strains, APEC isolated in the United States, UPEC (uropathogenic E. coli) and diarrheagenic strains isolated from humans. This correlation corroborates and reinforces the zoonotic potential hypothesis proposed to APEC.
Thesagro:	Escherichia Coli
Keywords:	Árvore filogenética Avian pathogenic Escherichia coli Tipagem de sequência multilocus Análise in silico Genes associados à virulência Multi-locus Sequence Typing Phylogenetic tree Virulence genes
DOI:	http://dx.doi.org/10.1590/S0100-736X2014000200006
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPTIA)