Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116740
Research center of Embrapa/Collection: Embrapa Pecuária Sudeste - Artigo em anais de congresso (ALICE)
Date Issued: 2019
Type of Material: Artigo em anais de congresso (ALICE)
Authors: GARCIA, A. R.
MOURA, A. B. B.
ROMANELLO, N.
FONSECA, J. F. da
BRANDÃO, F. Z.
PANTOJA, M. H. de A.
BARRETO, A. do N.
SOUSA, M. A. de P.
BOTTA, D.
GIRO, A.
Additional Information: ALEXANDRE ROSSETTO GARCIA, CPPSE; Ana Beatriz Bossois Moura, UFF; Narian Romanello, USP; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF; Messy Hannear de Andrade Pantoja, USP; Andréa do Nascimento Barreto, UFPA; Marco Antonio de Paula Sousa, UFPA; Daniela Botta, UFPA; Alessandro Giro, UFPA.
Title: Osmolaridade ideal para execução do teste hiposmótico no sêmen in natura de ovinos (Ovis aries).
Publisher: In: REUNIÃO ANUAL DA ASSOCIAÇÃO BRASILEIRA DE ANDROLOGIA ANIMAL (ABRAA), 4., 2019, Goiânia. Anais... Goiânia: Editora UFMS, 2019.
Pages: p.160-163.
Language: pt_BR
Notes: 7 e 8 de junho de 2019.
Keywords: Solução hiposmótica
Hypoosmotic solution
Ram
Sperm
Description: The study aimed to establish the most suitable osmolarity range of the hypoosmotic solution for testing the membrane integrity in raw semen of rams. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/L) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/L (S10). Nine mature Santa Ines rams (21.5+0.5 months; 72.9+1.6 kg) had semen collected one month apart. After the hypoosmotic swelling test (HOST) the ejaculates were evaluated using a phase-contrast microscope. A total of 200 spermatozoa were counted and sperm cells were classified as spermatozoa with coiled tails (CD, %), strongly coiled tail (CFD, %) or non-coiled tails. According to CD, S1, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P<0,05). According to CED, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P<0,05). Results suggest that the 75 up to 200 mOsm/L solution would be bestfor using in HOST when raw semen of rams is analyzed.
Thesagro: Ovino
Espermatozóide
Data Created: 2019-12-12
ISSN: 978-85-0000-000-0
Appears in Collections:Artigo em anais de congresso (CPPSE)

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