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|Research center of Embrapa/Collection:||Embrapa Florestas - Artigo em periódico indexado (ALICE)|
|Type of Material:||Artigo em periódico indexado (ALICE)|
|Authors:||GRUNENNVALDT, R. L.|
TOMASI, J. de C.
HANSEL, F. A.
GOMES, E. N.
|Additional Information:||RENATA LÚCIA GRUNENNVALDT, UFPR|
CÍCERO DESCHAMPS, UFPR.
JULIANA DEGENHARDT, CNPF
PETER BROOKS, University of The Sunshine Coast
JÉSSICA DE CÁSSIA TOMASI, UFPR
FABRICIO AUGUSTO HANSEL, CNPF
TRONG TRAN, University of The Sunshine Coast
ERIK N. GOMES, The State University of New Jersey
|Title:||Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype infl uence, medium optimization and compounds identifi cation.|
|Publisher:||Anais da Academia Brasileira de Ciências , v. 92, n. 3, e20181251, 2020. 16 p.|
Plant growth regulators
Acúmulo de biomassa
|Description:||Ilex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine.|
Regulador de Crescimento
|NAL Thesaurus:||Yerba mate|
|Appears in Collections:||Artigo em periódico indexado (CNPF)|
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|Juliana-Grunennvaldt-et-al-2020.pdf||2.4 MB||Adobe PDF|