Title:	Antitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines.
Authors:	NIGRA, A. D. GUIMAREAES, D. de A. B. PRUCCA, C. G. FREITAS-SILVA, O. TEODORO, A. J. GIL, G. A.
Affiliation:	AYELÉN D. NIGRA, Universidad Nacional de Córdoba; DEBORAH DE ALMEIDA BAUER GUIMARÃES, Universidade Federal do Estado do Rio de Janeiro -UNIRIO; CÉSAR G. PRUCCA, Universidad Nacional de Córdoba; OTNIEL FREITAS SILVA, CTAA; ANDERSON J. TEODORO, Universidade Federal do Estado do Rio de Janeiro - UNIRIO; GERMÁN A. GIL, Universidad Nacional de Córdoba.
Date Issued:	2021
Citation:	Oxidative Medicine and Cellular Longevity, 2021, ID 5572630, p. 1-16.
Description:	Coffee consumption is believed to have chemopreventive and chemotherapeutic effects and to contribute to preventing thedevelopment and progression of cancer. However, there is still controversy around these claims. As indicated in our previousworks, diet can influence the risk of breast cancer. Intake of coffee is hypothesized to reduce this risk, but current scientificevidence is not conclusive. This work is aimed at studying the effects of Robusta coffee bean extract on cell viability,proliferation, and apoptosis of different human cancers, especially breast cancer cell lines. To this end, cell viability wasevaluated by Alamar Blue in 2D and 3D models, the cell cycle by PI, apoptosis by annexin V, mitochondrial morphology, andfunctionality by mitoTracker, and colony formation capacity by the clonogenic assay. Green and dark coffee extract significantlyreduced viability in human breast, colorectal, brain, and bone cancer cells. Coffee anticancer activity was clearly evidenced inMDA-MB-231 (ER-) and MCF-7 (ER+) breast cancer cells but not in the normal breast cell line. In addition, coffee extractinduces an increase S phase and a decrease G2/M population in breast cancer cells, affected the mitochondrial morphology, andtriggered apoptosis. MDA-MB-231 breast cancer cells lost their clonogenic capacity after treatment. The antitumor activity wasdemonstrated in both 2D and 3D culture cell models
Thesagro:	Café Robusta Grão Extrato Vegetal Câncer Produto de Origem Vegetal
NAL Thesaurus:	Food technology
DOI:	https://doi.org/10.1155/2021/5572630
Notes:	Research Article.
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CTAA)