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Título: Development of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols.
Autor: MARGARIDO, A.
MANZINE, L. R.
ARAUJO-MOREIRA, F. M.
GONÇALVES, R. V.
HERRMANN JUNIOR, P. S. de P.
Afiliación: PAULO SERGIO DE P HERRMANN JUNIOR, CNPDIA.
Año: 2021
Referencia: Engineering Proceedings, v. 6, n. 75 2021.
Páginas: 1 - 5
Descripción: This paper describes the development of a biosensor designed for the enzymatic detection of short-chain alcohols. The biorecognition element, alcohol dehydrogenase, was immobilized on selfassembled monolayers deposited on top of silicon nitride microcantilevers. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. X-ray photoelectron spectroscopy and atomic force microscopy were used. The biosensor showed a lower response time and sensibility from 0.03 to 1.2 mL/L. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols and practically null involving other polar or nonpolar solvents.
Palabras clave: Biosensor
Microcantilever
Biorecognition element
ISSN: 2359-1757
DOI: https://doi.org/10.3390/I3S2021Dresden-10175
Notas: Presented at the 8th International Symposium on Sensor Science.
Tipo de Material: Artigo de periódico
Acceso: openAccess
Aparece en las colecciones:Artigo em periódico indexado (CNPDIA)

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