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http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134534
Título: | Development of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols. |
Autor: | MARGARIDO, A.![]() ![]() MANZINE, L. R. ![]() ![]() ARAUJO-MOREIRA, F. M. ![]() ![]() GONÇALVES, R. V. ![]() ![]() HERRMANN JUNIOR, P. S. de P. ![]() ![]() |
Afiliación: | PAULO SERGIO DE P HERRMANN JUNIOR, CNPDIA. |
Año: | 2021 |
Referencia: | Engineering Proceedings, v. 6, n. 75 2021. |
Páginas: | 1 - 5 |
Descripción: | This paper describes the development of a biosensor designed for the enzymatic detection of short-chain alcohols. The biorecognition element, alcohol dehydrogenase, was immobilized on selfassembled monolayers deposited on top of silicon nitride microcantilevers. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. X-ray photoelectron spectroscopy and atomic force microscopy were used. The biosensor showed a lower response time and sensibility from 0.03 to 1.2 mL/L. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols and practically null involving other polar or nonpolar solvents. |
Palabras clave: | Biosensor Microcantilever Biorecognition element |
ISSN: | 2359-1757 |
DOI: | https://doi.org/10.3390/I3S2021Dresden-10175 |
Notas: | Presented at the 8th International Symposium on Sensor Science. |
Tipo de Material: | Artigo de periódico |
Acceso: | openAccess |
Aparece en las colecciones: | Artigo em periódico indexado (CNPDIA)![]() ![]() |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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P-Development-of-an-Enzyme-Coated-Microcantilever-Based-....pdf | 590.03 kB | Adobe PDF | ![]() Visualizar/Abrir |