Title:	Phenomics-assisted selection for herbage accumulation in alfalfa (Medicago sativa L.).
Authors:	BISWAS, A. ANDRADE, M. H. M. L. ACHARYA, J. P. SOUZA, C. L. de LOPEZ, Y. ASSIS, G. M. L. de SHIRBHATE, S. SINGH, A. MUNOZ, P. RIOS, E. F.
Affiliation:	ANJU BISWAS, Department of Agronomy, University of Florida, Gainesville, FL, United States; MARIO HENRIQUE MURAD LEITE ANDRADE, Department of Agronomy, University of Florida, Gainesville, FL, United States; JANAM P. ACHARYA, Department of Agronomy, University of Florida, Gainesville, FL, United States; CLEBER LOPES DE SOUZA, Department of Agronomy, University of Florida, Gainesville, FL, United States; YOLANDA LOPEZ, Department of Agronomy, University of Florida, Gainesville, FL, United States; GISELLE MARIANO LESSA DE ASSIS, CPAF-AC; SHUBHAM SHIRBHATE, Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL, United States; ADITYA SINGH, Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL, United States; PATRICIO MUNOZ, Department of Horticultural Sciences, University of Florida, Gainesville, FL, United States; ESTEBAN F. RIOS, Department of Agronomy, University of Florida, Gainesville, FL, United States.
Date Issued:	2021
Citation:	Frontiers in Plant Science, v. 12, 756768, Dec. 2021.
Description:	The application of remote sensing in plant breeding is becoming a routine method for fast and non-destructive high-throughput phenotyping (HTP) using unmanned aerial vehicles (UAVs) equipped with sensors. Alfalfa (Medicago sativa L.) is a perennial forage legume grown in more than 30 million hectares worldwide. Breeding alfalfa for herbage accumulation (HA) requires frequent and multiple phenotyping efforts, which is laborious and costly. The objective of this study was to assess the efficiency of UAV-based imagery and spatial analysis in the selection of alfalfa for HA. The alfalfa breeding population was composed of 145 full-sib and 34 half-sib families, and the experimental design was a row-column with augmented representation of controls. The experiment was established in November 2017, and HA was harvested four times between August 2018 and January 2019. A UAV equipped with a multispectral camera was used for HTP before each harvest. Four vegetation indices (VIs) were calculated from the UAVbased images: NDVI, NDRE, GNDVI, and GRVI. All VIs showed a high correlation with HA, and VIs predicted HA with moderate accuracy. HA and NDVI were used for further analyses to calculate the genetic parameters using linear mixed models. The spatial analysis had a significant effect in both dimensions (rows and columns) for HA and NDVI, resulting in improvements in the estimation of genetic parameters. Univariate models for NDVI and HA, and bivariate models, were fit to predict family performance for scenarios with various levels of HA data (simulated in silico by assigning missing values to full dataset). The bivariate models provided higher correlation among predicted values, higher coincidence for selection, and higher genetic gain even for scenarios with only 30% of HA data. Hence, HTP is a reliable and efficient method to aid alfalfa phenotyping to improve HA. Additionally, the use of spatial analysis can also improve the accuracy of selection in breeding trials.
Thesagro:	Alfafa Medicago Sativa Leguminosa Forrageira Melhoramento Genético Vegetal Sensoriamento Remoto
NAL Thesaurus:	Plant breeding Phenotype Forage legumes Remote sensing Spatial variation
Keywords:	High-throughput phenotyping (HTP) Normalized difference vegetation index (NDVI) Genetic gain Fitomejoramiento Leguminosas forrajeras Teledetección Variación espacial
DOI:	https://doi.org/10.3389/fpls.2021.756768
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAF-AC)