Title:	Electrophoresis (SDS-PAGE) as a method for screening species of Passiflora using seed proteins as molecular markers.
Authors:	STEPHAN, M. P. COSTA, A. M. AZEVEDO, T. de L. ROSA, J. S. da SANTOS, A. A. dos
Affiliation:	MARILIA PENTEADO STEPHAN, CTAA; ANA MARIA COSTA, CPAC; TATIANA DE LIMA AZEVEDO, CTAA; JEANE SANTOS DA ROSA, CTAA; ALEXSANDRO ARAUJO DOS SANTOS, CTAA.
Date Issued:	2022
Citation:	Journal of Agricultural Science and Technology B, v. 12, n. 2, p. 40-47, 2022.
Description:	Brazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as an analysis tool for further screening of their protein profiles and selection of a molecular marker to differentiate them.
Thesagro:	Tecnologia de Alimento Método de Análise Eletroforese Purificação Proteína Peptídeo Fruto Maracujá Semente Casca Polpa Rastreamento
NAL Thesaurus:	Food technology Analytical methods Polyacrylamide gel electrophoresis Purification methods Proteins Peptides Fruits Passion fruits Pulp Passiflora Seeds Bioactive compounds
Keywords:	Bioactive peptides P setacea P tenuifila Mass distribution on fruit
DOI:	https://doi.org/10.17265/2161-6264/2022.02.002
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CTAA)