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http://www.alice.cnptia.embrapa.br/alice/handle/doc/1175097
Title: | Long-term preserved bean seeds exhibit high RNA integrity and high germination potential. |
Authors: | DANTAS, A. F.![]() ![]() NUNES, T. C. ![]() ![]() GRYNBERG, P. ![]() ![]() BRASILEIRO, A. C. M. ![]() ![]() SALOMAO, A. N. ![]() ![]() JOSE, S. C. B. R. ![]() ![]() VIANELLO, R. P. ![]() ![]() PADUA, J. G. ![]() ![]() PAPPAS, M. de C. R. ![]() ![]() GIMENES, M. A. ![]() ![]() |
Affiliation: | ALISSON FERREIRA DANTAS, NATIONAL COUNCIL FOR SCIENTIFIC AND TECHNOLOGICAL DEVELOPMENT; TAYARA COLINS NUNES, FEDERAL DISTRICT RESEARCH SUPPORT FOUNDATION; PRISCILA GRYNBERG, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; ANTONIETA NASSIF SALOMAO, CENARGEN; SOLANGE CARVALHO B ROVERI JOSE, CENARGEN; ROSANA PEREIRA VIANELLO, CNPAF; JULIANO GOMES PADUA, CENARGEN; MARILIA DE CASTRO RODRIGUES PAPPAS, CENARGEN; MARCOS APARECIDO GIMENES, CENARGEN. |
Date Issued: | 2025 |
Citation: | Scientific Reports, v.15, 12407, 2025. |
Description: | Stored RNA plays a key role in seed germination, especially after seeds long-term storage. This study aimed to assess the correlation between germination potential (GP) and RNA integrity in common bean (Phaseolus vulgaris L.) seed accessions stored at -18 °C for 19.25 to 43.75 years and in seeds subjected to artificial aging at high temperature (42 °C) and relative humidity (100%). The GP of long-term preserved and artificially aged seed lots ranged from 3 to 100% and 38–87%, respectively. RNA integrity was evaluated using the RNA Integrity Number (RIN, Agilent Bioanalyzer software). RIN data suggested that: (1) Sample sizes for RIN analysis may need to vary based on the GP of the seeds; (2) RIN has the potential to predict physiological quality, especially in seeds with high GP; (3) RIN values by itself may not accurately reflect the rate of RNA degradation over time, necessitating comparison with a control to determine ΔRIN (the difference between the RIN values of the sample and the control); (4) ΔRIN has a significant positive correlation with GP. These findings highlight the potential of RNA integrity in seeds as a molecular marker for developing tests that complement germination tests. |
Keywords: | Aged seeds Phaseolus vulgaris L RNA integrity number |
DOI: | https://doi.org/10.1038/s41598-024-85030-7 |
Type of Material: | Artigo de periódico |
Access: | openAccess |
Appears in Collections: | Artigo em periódico indexado (CENARGEN)![]() ![]() |
Files in This Item:
File | Description | Size | Format | |
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s41598-024-85030-7.pdf | 1.74 MB | Adobe PDF | ![]() View/Open |