Title:	Stomata counting automation facilitates C. canephora tetraploid selection.
Authors:	ANGELO, P. C. da S. CALOMENO, C. V. BERNARDES, A. L. A. ALMEIDA, G. T. S. ALMEIDA, L. E. F. QUINAGLIA, J. P. M. APRÍGIO, N. G. O. SERA, T. SHIGUEOKA, L. H. SERA, G. H.
Affiliation:	PAULA CRISTINA DA SILVA ANGELO, CNPCA; CELSO V. CALOMENO, FIOCRUZ PARANÁ; ANDRE L. A. BERNARDES; GABRIELA T. S. ALMEIDA, PIBIC IDR - PARANÁ; LARISSA E. F. ALMEIDA, PIBIC IDR - PARANÁ; JOÃO PEDRO M. QUINAGLIA, PIBIC IDR - PARANÁ; NICOLLAS G. O. APRÍGIO; TUMORU SERA; LUCIANA H. SHIGUEOKA; GUSTAVO H. SERA, IDR - PARANÁ.
Date Issued:	2025
Citation:	In: CONFERENCE OF ASSOCIATION FOR THE SCIENCE AND INFORMATION ON COFFEE, 30., 2025, Lisboa, Portugal. Book of abstracts.
Pages:	p. 123.
Description:	Chromosome doubling can enhance reproductive compatibility between genotypes with different ploidy levels. We ́ve been using anti-mitotics to double a C. canephora clone chromosome number in order to facilitate nematode resistance introgression to C. arabica cultivars. Tetraploid plants will be homozygous, and will be potentially able to contribute complete resistance gene sets for interspecific hybrid progenies. Once treated with anti-mitotics, the plants had the stomata counted as the initial screening for tetraploids. The chromosome number is inversely correlated to the stomata number in diploid and tetraploid C. canephora plants [1]. Stomata counting is, nevertheless, time consuming and monotonous. The objective of this work was to test a software tool to identify and quantify stomata and compare scores with human scores produced by different people.
Thesagro:	Coffea Canephora Coffea Arábica
Keywords:	Software Anti-mitotic
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (SAPC)