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Título: Primers annealing to the coding sequences can identify SH3 gene variants.
Autor: ANGELO, P. C. da S.
YAMAGISHI, M. E. B.
SHIGUEOKA, L. H.
SERA, G. H.
CAIXETA, E. T.
Afiliación: PAULA CRISTINA DA SILVA ANGELO, CNPCA
MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA
LUCIANA H. SHIGUEOKA, IDR - PARANÁ
GUSTAVO H. SERA, IDR - PARANÁ
EVELINE TEIXEIRA CAIXETA MOURA, CNPCA.
Año: 2025
Referencia: CONFERENCE OF ASSOCIATION FOR THE SCIENCE ANDA INFORMATIO ON COFFEE, 30., 2025, Lisboa, Portugal.
Descripción: SH3 resistance factor to the coffee leaf rust was found in C. liberica and has been valued because it is durable. Working in silico, we demonstrated that SH3 loci are complex in most species displaying four up to eight SH3-NBS LRR gene variants placed in chromosomes 3. One additional variant can be found isolated in chromosomes 6. We recently proposed structural models for six C. liberica SH3-Rx-CCNBS-LRR variants [1]. Among these, three are very similar to each other and seems exclusive to C. liberica. This work was directed to develop a primer system useful to identify those three variants (LIB2, 3 and 4), and demonstrate its presence/absence in genotypes with introgression from C. liberica.
NAL Thesaurus: Coffea liberica
DNA primers
Palabras clave: Gene SH3-NBS
Loci SH3
Tipo de Material: Resumo em anais e proceedings
Acceso: openAccess
Aparece en las colecciones:Resumo em anais de congresso (SAPC)

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