Title:	Primers annealing to the coding sequences can identify SH3 gene variants.
Authors:	ANGELO, P. C. da S. YAMAGISHI, M. E. B. SHIGUEOKA, L. H. SERA, G. H. CAIXETA, E. T.
Affiliation:	PAULA CRISTINA DA SILVA ANGELO, CNPCA MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA LUCIANA H. SHIGUEOKA, IDR - PARANÁ GUSTAVO H. SERA, IDR - PARANÁ EVELINE TEIXEIRA CAIXETA MOURA, CNPCA.
Date Issued:	2025
Citation:	In: CONFERENCE OF ASSOCIATION FOR THE SCIENCE AND INFORMATION ON COFFEE, 30., 2025, Lisboa. Book of abstracts.
Pages:	p. 82.
Description:	SH3 resistance factor to the coffee leaf rust was found in C. liberica and has been valued because it is durable. Working in silico, we demonstrated that SH3 loci are complex in most species displaying four up to eight SH3-NBS LRR gene variants placed in chromosomes 3. One additional variant can be found isolated in chromosomes 6. We recently proposed structural models for six C. liberica SH3-Rx-CCNBS-LRR variants [1]. Among these, three are very similar to each other and seems exclusive to C. liberica. This work was directed to develop a primer system useful to identify those three variants (LIB2, 3 and 4), and demonstrate its presence/absence in genotypes with introgression from C. liberica.
NAL Thesaurus:	Coffea liberica DNA primers
Keywords:	Gene SH3-NBS Loci SH3
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (SAPC)