Title:	Gene expression in oocytes treated with camp modulators during in vitro prematuration.
Authors:	MELO, A. M. de NASCIMENTO, B. dos S. PEREIRA, H. P. AL SHEBL, W. QUINTAO, C. C. R. OLIVEIRA, C. S. GARCIA, J. M. SARAIVA, N. Z.
Affiliation:	ALEXANDRE MARCOS DE MELO; BEATRIZ DOS SANTOS NASCIMENTO, UNIVERSIDADE FEDERAL FLUMINENSE; HYAGO PASSE PEREIRA; WASIM AL SHEBL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; CLARA SLADE OLIVEIRA, CNPGL; JOAQUIM MANSANO GARCIA, UNIVERSIDADE ESTADUAL PAULISTA; NAIARA ZOCCAL SARAIVA, CNPGL.
Date Issued:	2025
Citation:	In: INTERNATIONAL CONGRESS OF THE BRAZILIAN GENETICS SOCIETY, 70., 2025, Belém. E-book: [abstracts]. Ribeirão Preto: Sociedade Brasileira de Genética, 2025.
Pages:	p. 233.
Description:	In vitro maturation (IVM) success in bovine oocytes is critically limited by intrinsic developmental competence. Modulation of cyclic adenosine monophosphate (cAMP) signaling pathways has emerged as a promising approach to enhance oocyte quality. This study aimed to evaluate the molecular effects of prematuration using cAMP modulators during IVM in a simulated physiological oocyte maturation (SPOM) system, assessing competence through gene expression analysis. Oocytes were matured in vitro using TCM-199 medium supplemented with 10% fetal bovine serum (FBS). The treatment group was exposed initially to 100 µM forskolin and 750 µM IBMX for 2 hours, then transferred to a basal medium containing 20 µM cilostamide for up to 28 hours.
Thesagro:	Bovino Melhoramento Genético Animal
Keywords:	Expressão gênica GDF9
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CNPGL)