Title:	Purificação de IgG de codorna a partir de soro e gema de ovos.
Authors:	OLIVEIRA, D. D. RESENDE, J. S. FLATSCHAR, A. V. F. ABREU, J. T. FLATSCHART, R. B. MARTINS, N. R. S.
Affiliation:	AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS.
Date Issued:	2010
Citation:	Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 62, n. 2, p. 492-494, 2010.
Description:	The purification of Japanese quail IgG from serum was performed using caprilic acid and ammonium sulfate, and from egg yolk using PEG-6000 and ethanol. After confirming the purification and the concentration of IgG, the yolk samples had twice the amount of protein compared to serum samples. The IgG extracts were analyzed by SDS-PAGE and western blot showing similar results as the ones of chicken IgG used as the standard. So, these methodologies can be used for purifying quail serum or egg yolk IgG, which would enable the development of diagnostic assays.
Thesagro:	Imunoglobulina Soro
DOI:	10.1590/S0102-09352010000200034
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMS)