Title:	Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers.
Authors:	SANTOS, C. A. F. LIMA NETO, F. P.
Affiliation:	CARLOS ANTONIO FERNANDES SANTOS, CPATSA; FRANCISCO PINHEIRO LIMA NETO, CPATSA.
Date Issued:	2011
Citation:	Pesquisa Agropecuária Brasileira, v. 46, n.8, p. 899-904, ago. 2011
Description:	The objective of this work was to estimate outcrossing rates between Haden and Tommy Atkins mango cultivars, using AFLP and microsatellite markers. Progenies of an isolated 'Haden' plant, identified in a 'Tommy Atkins' commercial orchard, in Petrolina, PE, Brazil, were analyzed. Total DNA was isolated from the progeny leaves and used for AFLP and microsatellite reactions. Multilocus outcrossing rates (t m) were estimated by direct count of AFLP or microsatellite markers and by the mLTR software. Outcrossing rates ranged from 0.85 to 0.87 with the analysis based on seven AFLP markers, and from 0.83 to 0.91 based on three microsatellite primers. No unexpected band patterns were observed for 'Haden' and 'Tommy Atkins'. The estimates obtained with the mLTR software were close to those obtained by direct AFLP and microsatellite allele counting, which indicates that the multilocus model was appropriate for this kind of study. The microsatellites mMiCIR005, mMiCIR030, and mMiCIR036 can be used to elucidate the origin of 'Haden' and 'Tommy Atkins' seedlings
Thesagro:	Manga Mangifera Indica Melhoramento Genético Vegetal
NAL Thesaurus:	Molecular genetics Mangoes
Keywords:	MLTR Estimativa multilocos SSR Haden Tommy Atkins Marcadores microssatélites AFLP
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPATSA)