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Título: Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers.
Autor: SANTOS, C. A. F.
LIMA NETO, F. P.
Afiliación: CARLOS ANTONIO FERNANDES SANTOS, CPATSA; FRANCISCO PINHEIRO LIMA NETO, CPATSA.
Año: 2011
Referencia: Pesquisa Agropecuária Brasileira, v. 46, n.8, p. 899-904, ago. 2011
Descripción: The objective of this work was to estimate outcrossing rates between Haden and Tommy Atkins mango cultivars, using AFLP and microsatellite markers. Progenies of an isolated 'Haden' plant, identified in a 'Tommy Atkins' commercial orchard, in Petrolina, PE, Brazil, were analyzed. Total DNA was isolated from the progeny leaves and used for AFLP and microsatellite reactions. Multilocus outcrossing rates (t m) were estimated by direct count of AFLP or microsatellite markers and by the mLTR software. Outcrossing rates ranged from 0.85 to 0.87 with the analysis based on seven AFLP markers, and from 0.83 to 0.91 based on three microsatellite primers. No unexpected band patterns were observed for 'Haden' and 'Tommy Atkins'. The estimates obtained with the mLTR software were close to those obtained by direct AFLP and microsatellite allele counting, which indicates that the multilocus model was appropriate for this kind of study. The microsatellites mMiCIR005, mMiCIR030, and mMiCIR036 can be used to elucidate the origin of 'Haden' and 'Tommy Atkins' seedlings
Thesagro: Manga
Mangifera Indica
Melhoramento Genético Vegetal
NAL Thesaurus: Molecular genetics
Mangoes
Palabras clave: MLTR
Estimativa multilocos
SSR
Haden
Tommy Atkins
Marcadores microssatélites
AFLP
Tipo de Material: Artigo de periódico
Acceso: openAccess
Aparece en las colecciones:Artigo em periódico indexado (CPATSA)

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