Title:	Finding protein-protein interaction patterns by contact map matching.
Authors:	MELO, R. C. RIBEIRO, C. MURRAY, C. S. VELOSO, C. J. M. SILVEIRA, C. H. da NESHICH, G. MEIRA JUNIOR, W. CARCERONI, R. L. SANTORO, M. M.
Affiliation:	UFMG; UFMG; UFMG; UFMG; UFMG; GORAN NESHICH, CNPTIA; UFMG; UFMG; UFMG.
Date Issued:	2007
Citation:	Genetics and Molecular Research, v. 6, n. 4, p. 946-963, 2007.
Description:	We propose a novel method for defining patterns of contacts present in protein-protein complexes. A new use of the traditional contact maps (more frequently used for representation of the intra-chain contacts) is presented for analysis of inter-chain contacts. Using an algorithm based on image processing techniques, we can compare protein-protein interaction maps and also obtain a dissimilarity score between them. The same algorithm used to compare the maps can align the contacts of all the complexes and be helpful in the determination of a pattern of conserved interactions at the interfaces. We present an example for the application of this method by analyzing the pattern of interaction of bovine pancreatic trypsin inhibitors and trypsins, chymotrypsins, a thrombin, a matriptase, and a kallikrein - all classified as serine proteases. We found 20 contacts conserved in trypsins and chymotrypsins and 3 specific ones are present in all the serine protease complexes studied. The method was able to identify important contacts for the protein family studied and the results are in agreement with the literature.
NAL Thesaurus:	Bioinformatics Serine proteinases protein-protein interactions
Keywords:	Bioinformática Interação proteína-proteína Mapas de contato Contact maps Serine proteases
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPTIA)