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Título: Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack.
Autor: MIRANDA, V. de J.
COELHO, R. R.
VIANA, A. A. B.
OLIVEIRA NETO, O. B. de
CARNEIRO, R. M. D. G.
ROCHA, T. L.
SA, M. F. G. de
FRAGOSO, R. da R.
Afiliación: VÍVIAN DE JESUS MIRANDA
ROBERTA RAMOS COELHO
ANTÔNIO AMÉRICO BARBOSA VIANA
OSMUNDO BRILHANTE DE OLIVEIRA NETO
REGINA MARIA DECHECHI G CARNEIRO, CENARGEN
THALES LIMA ROCHA, CENARGEN
MARIA FATIMA GROSSI DE SA, CENARGEN
RODRIGO DA ROCHA FRAGOSO, CPAC.
Año: 2013
Referencia: BMC Research Notes, v. 6, n. 196, 2013.
Descripción: Soybean pathogens and pests reduce grain production worldwide. Biotic interaction cause extensive changes in plant gene expression profile and the data produced by functional genomics studies need validation, usually done by quantitative PCR. Nevertheless, this technique relies on accurate normalization which, in turn, depends upon the proper selection of stable reference genes for each experimental condition. To date, only a few studies were performed to validate reference genes in soybean subjected to biotic stress. Here, we report reference genes validation in soybean during root-knot nematode (Meloidogyne incognita) parasitism and velvetbean caterpillar (Anticarsia gemmatalis) attack.
Thesagro: Genética
Praga de Planta
Soja
Palabras clave: Patógenos
Genes de referencia
Tipo de Material: Artigo de periódico
Acceso: openAccess
Aparece en las colecciones:Artigo em periódico indexado (CPAC)

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