Título:	Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack.
Autoria:	MIRANDA, V. de J. COELHO, R. R. VIANA, A. A. B. OLIVEIRA NETO, O. B. de CARNEIRO, R. M. D. G. ROCHA, T. L. SA, M. F. G. de FRAGOSO, R. da R.
Afiliação:	VÍVIAN DE JESUS MIRANDA ROBERTA RAMOS COELHO ANTÔNIO AMÉRICO BARBOSA VIANA OSMUNDO BRILHANTE DE OLIVEIRA NETO REGINA MARIA DECHECHI G CARNEIRO, CENARGEN THALES LIMA ROCHA, CENARGEN MARIA FATIMA GROSSI DE SA, CENARGEN RODRIGO DA ROCHA FRAGOSO, CPAC.
Ano de publicação:	2013
Referência:	BMC Research Notes, v. 6, n. 196, 2013.
Conteúdo:	Soybean pathogens and pests reduce grain production worldwide. Biotic interaction cause extensive changes in plant gene expression profile and the data produced by functional genomics studies need validation, usually done by quantitative PCR. Nevertheless, this technique relies on accurate normalization which, in turn, depends upon the proper selection of stable reference genes for each experimental condition. To date, only a few studies were performed to validate reference genes in soybean subjected to biotic stress. Here, we report reference genes validation in soybean during root-knot nematode (Meloidogyne incognita) parasitism and velvetbean caterpillar (Anticarsia gemmatalis) attack.
Thesagro:	Genética Praga de Planta Soja
Palavras-chave:	Patógenos Genes de referencia
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPAC)