Título:	Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.
Autoria:	MUNIZ, C. R. SILVA, G. F. da SOUZA JUNIOR, M. T. FREIRE, F. das C. O. KEMA, G. H. J. GUEDES, M. I. F.
Afiliação:	CELLI RODRIGUES MUNIZ, CNPAT; GILVAN FERREIRA DA SILVA, CPAA; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; G. H. J. Kema, Biointeractions and Plant Health; M. I. F. Guedes, Universidade Estadual do Ceará.
Ano de publicação:	2014
Referência:	Genetics and Molecular Research, v. 13, n. 2, p. 2906-2913, 2014.
Conteúdo:	Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation.
Thesagro:	Agrobacterium Tumefaciens Lasiodiplodia Theobromae
NAL Thesaurus:	gummosis
Palavras-chave:	Cashew plants Mediated transformation
Digital Object Identifier:	DOI http://dx.doi.org/10.4238/2014.February.21.8
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPAA)