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Protocol for the establishment of a serine integrase-based platform for functional validation of genetic switch controllers in eukaryotic cells.
Serine integrases (Ints) are a family of site-specific recombinases (SSRs) encoded by some bacteriophages to integrate their genetic material into the genome of a host. Their ability to rearrange DNA sequences in different ways including inversion, excision, or insertion with no help fr...
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