Title:	Molecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the Order Picornavirales.
Authors:	NERY, F. M. B. MELO, F. L. BOITEUX, L. S. RIBEIRO, S. da G. RESENDE, R. O. ORÍLIO, A. F. BATISTA, J. G. LIMA, M. F. PEREIRA-CARVALHO, R. C.
Affiliation:	FLÁVIA M. B. NERY, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; LEONARDO SILVA BOITEUX, CNPH; SIMONE DA GRACA RIBEIRO, Cenargen; RENATO O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANELISE F. ORÍLIO, UNIVERSIDADE DE BRASÍLIA; JOSIANE G. BATISTA, UNIVERSIDADE DE BRASÍLIA; MIRTES FREITAS LIMA, CNPH; RITA C. PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA.
Date Issued:	2020
Citation:	Viruses, v. 12, n. 9, 950, 2020
Description:	In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.
Thesagro:	Hovenia Dulcis
NAL Thesaurus:	Viruses Metagenomics Picornavirales
Keywords:	HDaV1 HDaV2
DOI:	https://doi.org/10.3390/v12090950
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPH)