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|Research center of Embrapa/Collection:||Embrapa Meio Ambiente - Artigo em periódico indexado (ALICE)|
|Type of Material:||Artigo em periódico indexado (ALICE)|
|Authors:||MEDEIROS, A. M. Z.|
KHAN, L. U.
SILVA, G. H. da
OSPINA, C. A.
ALVES, O. L.
CASTRO, V. L. S. S. de
MARTINEZ, D. S. T.
|Additional Information:||ALINE MARIA ZIGIOTTO DE MEDEIROS, CNPEM; LATIF ULLAH KHAN, CNPEM; GABRIELA HELENA DA SILVA, CNPEM; CARLOS ALBERTO OSPINA, CNPEM; OSWALDO LUIZ ALVES, IQ-UNICAMP; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; DIEGO STÉFANI TEODORO MARTINEZ, CNPEM.|
|Title:||Graphene oxide-silver nanoparticle hybrid material: an integrated nanosafety study in zebrafish embryos.|
|Publisher:||Ecotoxicology and Environmental Safety, v. 209, article 111776, 2021.|
|Description:||Abstract: This work reports an integrated nanosafety study including the synthesis and characterization of the graphene oxide-silver nanoparticle hybrid material (GO-AgNPs) and its nano-ecotoxicity evaluation in the zebrafish embryo model. The influences of natural organic matter (NOM) and a chorion embryo membrane were considered in this study, looking towards more environmentally realistic scenarios and standardized nanotoxicity testing. The nanohybrid was successfully synthesized using the NaBH4 aqueous method, and AgNPs (~ 5.8 nm) were evenly distributed over the GO surface. GO-AgNPs showed a dose-response acute toxicity: the LC50 was 1.5 mg L-1 for chorionated embryos. The removal of chorion, however, increased this toxic effect by 50%. Furthermore, the presence of NOM mitigated mortality, and LC50 for GO-AgNPs changed respectively from 2.3 to 1.2 mg L-1 for chorionated and de-chorionated embryos. Raman spectroscopy confirmed the ingestion of GO by embryos; but without displaying acute toxicity up to 100 mg L-1, indicating that the silver drove toxicity down. Additionally, it was observed that silver nanoparticle dissolution has a minimal effect on these observed toxicity results. Finally, understanding the influence of chorion membranes and NOM is a critical step towards the standardization of testing for zebrafish embryo toxicity in safety assessments and regulatory issues.|
|Thesagro:||Composto de Carbono|
Peixe de Água Doce
|Appears in Collections:||Artigo em periódico indexado (CNPMA)|
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|Castro-Graphene-oxide-2021.pdf||14,06 MB||Adobe PDF|