Title:	Graphene oxide-silver nanoparticle hybrid material: an integrated nanosafety study in zebrafish embryos.
Authors:	MEDEIROS, A. M. Z. KHAN, L. U. SILVA, G. H. da OSPINA, C. A. ALVES, O. L. CASTRO, V. L. S. S. de MARTINEZ, D. S. T.
Affiliation:	ALINE MARIA ZIGIOTTO DE MEDEIROS, CNPEM; LATIF ULLAH KHAN, CNPEM; GABRIELA HELENA DA SILVA, CNPEM; CARLOS ALBERTO OSPINA, CNPEM; OSWALDO LUIZ ALVES, IQ-UNICAMP; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; DIEGO STÉFANI TEODORO MARTINEZ, CNPEM.
Date Issued:	2021
Citation:	Ecotoxicology and Environmental Safety, v. 209, article 111776, 2021.
Description:	Abstract: This work reports an integrated nanosafety study including the synthesis and characterization of the graphene oxide-silver nanoparticle hybrid material (GO-AgNPs) and its nano-ecotoxicity evaluation in the zebrafish embryo model. The influences of natural organic matter (NOM) and a chorion embryo membrane were considered in this study, looking towards more environmentally realistic scenarios and standardized nanotoxicity testing. The nanohybrid was successfully synthesized using the NaBH4 aqueous method, and AgNPs (~ 5.8 nm) were evenly distributed over the GO surface. GO-AgNPs showed a dose-response acute toxicity: the LC50 was 1.5 mg L-1 for chorionated embryos. The removal of chorion, however, increased this toxic effect by 50%. Furthermore, the presence of NOM mitigated mortality, and LC50 for GO-AgNPs changed respectively from 2.3 to 1.2 mg L-1 for chorionated and de-chorionated embryos. Raman spectroscopy confirmed the ingestion of GO by embryos; but without displaying acute toxicity up to 100 mg L-1, indicating that the silver drove toxicity down. Additionally, it was observed that silver nanoparticle dissolution has a minimal effect on these observed toxicity results. Finally, understanding the influence of chorion membranes and NOM is a critical step towards the standardization of testing for zebrafish embryo toxicity in safety assessments and regulatory issues.
Thesagro:	Composto de Carbono Toxidez Peixe de Água Doce
NAL Thesaurus:	Ecotoxicology Graphene oxide Nanomaterials Danio rerio chorion
Keywords:	Nanohybrids Nanoecotoxicology Nanotoxicity Alternative methods Grafeno Nanomateriais
ISSN:	0147-6513
DOI:	https://doi.org/10.1016/j.ecoenv.2020.111776
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPMA)