Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1132164
Research center of Embrapa/Collection: Embrapa Soja - Capítulo em livro científico (ALICE)
Date Issued: 2021
Type of Material: Capítulo em livro científico (ALICE)
Authors: VIEIRA, L. R.
FREITAS, N. C.
JUSTEN, F.
MIRANDA, V. de J.
GARCIA, B. de O.
NEPOMUCENO, A. L.
FUGANTI-PAGLIARINI, R.
FELIPE, M. S. S.
MOLINARI, H. B. C.
VELINI, E. D.
PINTO, E. R. de C.
DAGLI, M. L. Z.
ANDRADE, G.
FERNANDES, P. M. B.
MERTZ-HENNING, L. M.
KOBAYASHI, A. K.
Additional Information: LETÍCIA RIOS VIEIRA; NATÁLIA CHAGAS FREITAS; FERNANDA JUSTEN; VÍVIAN DE JESUS MIRANDA; BRUNO DE OLIVEIRA GARCIA, Universidade Federal de Lavras; ALEXANDRE LIMA NEPOMUCENO, CNPSO; RENATA FUGANTI-PAGLIARINI; MARIA SUELI SOARES FELIPE, Universidade Católica de Brasília; HUGO BRUNO CORREA MOLINARI, CNPAE; EDIVALDO DOMINGUES VELINI, Universidade Estadual Paulista; EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; MARIA LUCIA ZAIDAN DAGLI, Universidade de São Paulo; GALDINO ANDRADE, Universidade Estatual de Londrina; PATRICIA MACHADO BUENO FERNANDES, Universidade Federal do Espírito Santo; LILIANE MARCIA MERTZ HENNING, CNPSO; ADILSON KENJI KOBAYASHI, CNPAE.
Title: Regulatory framework of genome editing in Brazil and worldwide.
Publisher: In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021. Chapter 5, p. 169-195.
Language: Ingles
Description: The regulation of the use of products obtained through genome-editing techniques has been the subject of great debate worldwide. Currently, the discussions are mainly focused on whether products obtained by different strategies of site-directed nucleases (SDN) should or not be classified as Genetically Modified Organisms (GMOs). In the SDN-1 application, the natural DNA cell repair pathway (Non-Homologous End-Joining - NHEJ) is explored to introduce simple random mutations (substitutions, insertions, and deletions) by systems such as CRISPR-Cas, TALENs, or Zinc Fingers Nucleases, which cause silencing of the gene product after breaking DNA (by Double-Strand Break - DSB). In the SDN-2 approach, a template DNA is also used to introduce a change in the sequence of nitrogen bases (A, C, G, T) at the target site where the DSB occurred, exploring another natural repair system directed by a DNA fragment from the same species (Homology-Directed Repair - HDR). In the SDN-3 approach, both NHEJ and HDR can be explored to insert one or more DNA fragments with sequences necessary for the expression of a gene (promoter, coding, and terminator region) at a specific location in the genome. In the following topics, questions related to genome editing regulation in different countries are discussed in detail.
Thesagro: Biotecnologia
DNA
Engenharia Genética
Genética Vegetal
Legislação
NAL Thesaurus: Biotechnology
Genetic engineering
Genetically modified organisms
Plant genetics
Data Created: 2021-06-02
Appears in Collections:Capítulo em livro científico (CNPSO)

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