Title:	Effect of different protocols for cryopreservation of skin fibroblasts from Sindi dairy bull.
Authors:	RODRIGUES, F. L. OLIVEIRA, I. R. dos S. MELO, J. C. de SILVA, J. R. S. da FERREIRA, I. C. MARTINS, C. F.
Affiliation:	FABIANA LIMA RODRIGUES, CENTRO UNIVERSITÁRIO ICESP; ISABELLA RODRIGUES DOS SANTOS OLIVEIRA, UNIVERSIDADE DE BRASÍLIA; JULIA CARVALHO DE MELO, UNIVERSIDADE DE BRASÍLIA; JOSÉ RICARDO SOBRAL DA SILVA, CENTRO UNIVERSITÁRIO DO PLANALTO CENTRAL; ISABEL CRISTINA FERREIRA, CPAC; CARLOS FREDERICO MARTINS, CPAC.
Date Issued:	2025
Citation:	Animal Reproduction, v. 22, n. 3, 2025.
Description:	The establishment of a germplasm bank using somatic cells is an important step prior to the use of genetic material in the bovine cloning process. Considering that each freezing curve temperature has specific implications for cell viability and functionality, the objective of this study was to evaluate the effect of three freezing protocols for fibroblasts from a high-genetic-merit dairy Sindi bull intended for cloning: 1. Pre-freezing at -80°C for 24 hours followed by immersion in liquid nitrogen (LN2); 2. Pre-freezing at -20°C for 24 hours followed by immersion in LN2; 3. Cooling to 5°C for 1 hour, gradual freezing in LN2 vapor for 20 minutes, and storage in LN2.
Thesagro:	Banco de Germoplasma Bovino Gado Sindi Clonagem Criopreservação Touro
NAL Thesaurus:	Cryopreservation Fibroblasts Dairy bulls Skin (animal) Somatic cells
Notes:	Edição dos Proceedings 38th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2025.
Type of Material:	Resumo em anais e proceedings
Access:	openAccess
Appears in Collections:	Resumo em anais de congresso (CPAC)