Title:	Protoplast production from napier grass and pearl millet triploid hybrids.
Authors:	TIMBÓ, A. L. de O. DAVIDE, L. C. PINTO, J. E. B. P. PEREIRA, A. V.
Affiliation:	ANA LUIZA DE OLIVEIRA TIMBÓ, UFLA; LISETE CHAMA DAVIDE, UFLA; JOSÉ EDUARDO BRASIL PEREIRA PINTO, UFLA; ANTONIO VANDER PEREIRA, CNPGL.
Date Issued:	2010
Citation:	Ciência e Agrotecnologia, v. 34, n. 5, p. 1219-1223, set./out. 2010.
Description:	The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time.
Thesagro:	Pennisetum Glaucum Pennisetum Purpureum
NAL Thesaurus:	forage
Keywords:	In vitro cultivation Interspecific hybrids
DOI:	https://doi.org/10.1590/S1413-70542010000500020
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CNPGL)